Application

Anti-GATA2 Antibody, clone 3C10.1, Cat. No. MABS1814, is a mouse monoclonal antibody that targets GATA2 and has been tested in Western Blotting.

General description

Endothelial transcription factor GATA-2 (UniProt: P23769; also known as GATA-binding protein 2) is encoded by the GATA2 gene (Gene ID: 2624) in human. GATA2 is a zinc-finger transcriptional activator that regulates endothelin-1 gene expression in endothelial cells. It binds to the consensus sequence 5′-AGATAG-3′. GATA2 is expressed in embryonic brain and liver, endothelial cells, and in erythroid cells. GATA2 mutations appear to cause loss of function of the mutated allele leading to haploinsufficiency, which results in immunodeficiency characterized by significantly reduced or absent monocytes, B-lymphocytes, natural killer lymphocytes, and circulating and tissue dendritic cells. However, it does not affect the T-cell number. This deficiency leads to increased susceptibility to mycobacterial, papillomavirus, and opportunistic fungal infections. Certain mutations in GATA2 are also linked to lymphedema that results in swelling of the extremities due to altered lymphatic flow.

Immunogen

GST/His-tagged recombinant fragment corresponding to 114 amino acids from the internal region of human GATA2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GATA2 in 10 µg of SH-SY5Y cell lysate.

Specificity

Clone 3C10.1 specifically detects Endothelial transcription factor GATA-2 in human. It targets an epitope within 114 amino acids from the internal region.

Target description

~52 kDa observed; 50.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).